gabaγ2 receptor subunit Search Results


gabaγ2 receptor subunit  (Proteintech)
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Proteintech gabaγ2 receptor subunit
Figure 5. Fmr1 KO males have increased GABAergic innervation. (a) GABAA receptors in the POMC neuron soma were quantified; (b) synaptic GABAA receptors were quantified as appositions of <t>GABAγ2</t> and VGAT in POMC neurons. GABAA (red), VGAT (green), POMC (grey). At least 20 neurons per mouse were quantified and the average for each mouse was represented with a point, while bars present genotype average. Bottom, 3D reconstruction of confocal images using Imaris. Statistical significance, indicated with a * (p < 0.05) was determined with t-test followed by Tukey’s post hoc test.
Gabaγ2 Receptor Subunit, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 5. Fmr1 KO males have increased GABAergic innervation. (a) GABAA receptors in the POMC neuron soma were quantified; (b) synaptic GABAA receptors were quantified as appositions of GABAγ2 and VGAT in POMC neurons. GABAA (red), VGAT (green), POMC (grey). At least 20 neurons per mouse were quantified and the average for each mouse was represented with a point, while bars present genotype average. Bottom, 3D reconstruction of confocal images using Imaris. Statistical significance, indicated with a * (p < 0.05) was determined with t-test followed by Tukey’s post hoc test.

Journal: Scientific reports

Article Title: Increased body weight in mice with fragile X messenger ribonucleoprotein 1 (Fmr1) gene mutation is associated with hypothalamic dysfunction.

doi: 10.1038/s41598-023-39643-z

Figure Lengend Snippet: Figure 5. Fmr1 KO males have increased GABAergic innervation. (a) GABAA receptors in the POMC neuron soma were quantified; (b) synaptic GABAA receptors were quantified as appositions of GABAγ2 and VGAT in POMC neurons. GABAA (red), VGAT (green), POMC (grey). At least 20 neurons per mouse were quantified and the average for each mouse was represented with a point, while bars present genotype average. Bottom, 3D reconstruction of confocal images using Imaris. Statistical significance, indicated with a * (p < 0.05) was determined with t-test followed by Tukey’s post hoc test.

Article Snippet: Whole cell lysates were obtained from the dissected hypothalami from WT controls and FMR1 knockout mice and after protein determination, the same amount of protein from each sample was resolved on SDS-PAGE, transferred on nitrocellulose membrane and probed for: GABAγ2 receptor subunit (1:1000, 14104-1-AP, Proteintech), VGAT (1:1000, 131 011, Synaptic Systems), MC4R (1:1000, ab24233, Abcam) or β-tubulin (1:1000, sc-9104, Santa Cruz Biotechnology) (Supplementary information).

Techniques: